ACLY Knockout cell line(PANC-1)

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location： Home > Products > KO Cell Lines > ACLY Knockout cell line(PANC-1)

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ACLY Knockout cell line(PANC-1) Order

ACLY Knockout cell line(PANC-1)
Catalog#
YKO-H555
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ACLY Knockout cell line(PANC-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ACLY Knockout cell line (Panc-1)
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Gene Name
ACLY
Gene ID
47
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript ACLY-201)
Validation: PCR+Sanger Sequencing
ACLY Knockout cell line(PANC-1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Panc-1		 STR Info (Cell bank)
Cell Line:PANC-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 11111111
D7S820 810810
D16S539 11111111
VWA 15151515
TH01 7878
AMEL XXXX
TPOX 811811
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ACLY gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Panc-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ACLY gene knockout ACLY Knockout cell line (Panc-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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